<a href='https://brc.riken.jp/mus/pcr04906'>Genotyping protocol -PCR-</a> D1.129P2(Cg)-Cxcr4<tm1Yiw> Tg(Lck-cre)1Jtak D1.129P2(Cg)-Cxcr4<tm1Yiw> Tg(Lck-cre)1Jtak RBRC04906 Cxcr4 floxed mice were developed by Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo in 2010. Lck-cre Tg mice were developed by Junji Takeda, Osaka University in 1998. Double mutant mice were backcrossed DBA/1 for at least 8 times. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Arthritis Res. Ther., 12, R188 (2010). Eur. J. Immunol., 28(7):2159-2166 (1998).Please obtain the written consent from Professor Yoichiro Iwakura (iwakura@rs.tus.ac.jp; Research Institute for Biological Sciences, Tokyo University of Science) for the use. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. true Cxcr4 : 2010年 東京大学医科学研究所 岩倉洋一郎先生らにより開発。 E14.1 ES細胞由来。 Lck-cre : 1998年 大阪大学微生物病研究所 竹田潤二先生らにより開発。DBA/1Jに8世代戻し交配 (兄妹交配9代) (BRC寄託時)。 KO : 129P2/OlaHsd (E14.1) ; Tg : Not Specified ケモカイン受容体CXCR4遺伝子座にloxP配列が挿入されているマウス。Lck-Creアレルを持つ。 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Arthritis Res. Ther., 12, R188 (2010). Eur. J. Immunol., 28(7):2159-2166 (1998).<br>利用者は事前に岩倉洋一郎教授（iwakura@rs.tus.ac.jp；東京理科大学生命医科学研究所）にコンタクトをとり、利用の承諾を得ること。 DBA/1JJmsSlc DBA/1JJmsSlc Yoichiro IWAKURA D1-Cxcr4 floxed/LckCre Tg D1-Cxcr4 floxed/LckCre Tg C（3〜6か月） C (3-6 months) phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Cxcr4 genomic DNA, mouse Lck promoter, phage P1 Cre recombinase gene Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Cre/loxP system 岩倉　洋一郎 E14.1 [129P2/OlaHsd] Cxcr4 : 2010年 東京大学医科学研究所 岩倉洋一郎先生らにより開発。E14.1 ES細胞由来。Lck-cre : 1998年 大阪大学微生物病研究所 竹田潤二先生らにより開発。DBA/1Jに8世代戻し交配 (兄妹交配9代) (BRC寄託時)。KO : 129P2/OlaHsd (E14.1) ; Tg : Not Specified Cxcr4 floxed mice with Lck-cre transgene.